SearchLight Protein Array Technology is a multiplexing sandwich-ELISA system based on chemiluminescent or fluorescent detection of analytes whose respective capture-antibodies are spotted in arrays within each well of a 96-well microplate. As many as 16 analytes (4 x 4 array in each well) can be measured per well, meaning that 16 cytokines or other biomarkers can be assayed simultaneously with each 50 µl sample!

• Sensitive multiplex analysis – obtain more information per limited amount of valuable sample. Measure up to 16 cytokines or biomarkers per microplate well (i.e., per 50 µl sample), detecting even low-abundance proteins with the sensitivity afforded by quantitative chemiluminescence.
• Sample compatibility – SearchLight Technology has been used with a variety of sample types, including culture supernatant, serum, plasma, BAL, CSF, crevicular fluid, amniotic fluid, nasal wash, cell lysate, peritoneal lavage, synovial fluid, breath condensate, brain microdialysate and various tissue homogenates.
• Large analyte menu – antibodies for more than 300 cytokines and biomarkers have been tested and validated for use in the SearchLight Multiplex Platform. The menu of proteins includes assays for human, mouse, rat, non-human primate, canine, porcine and bovine samples.
• Fast and accurate imaging and analysis – CCD imaging of SearchLight Assays is completed in ~1 to 5 minutes, depending on the imaging system use, making it easy to share instrumentation and analyze multiple plates per day. SearchLight Array Analysis Software is validated for the technology. The image from each plate can be saved for a permanent record of raw experimental data.
• Flexibility – invest in the necessary imaging and software systems or use our convenient sample testing service. Work with us to design a custom array or use one of our many standard array kits.